Hosuk:LabNotes/2014-9-5

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Revision as of 20:19, 7 September 2014 by >Hosuki78
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PGP1F 2nd rolonyar20140414 – Decode again with 63x

  • 63x, zoom=1, 2x2 tile (each tile index : s0~s3, --> converted to s1~s4 for analysis)
  • Z=0.49um, ~22 images


Result

  • Image signal were too saturated… need to use less PMT gain… (this time it was 660, 4% laser)


Merged and each Tile image of BF, 1st rolonies

File:Merged Tile BF.png File:Merged Tile 1stRolony.png


Mapped table and intensity plot

File:GeneMapTable IntPlot s1.png File:GeneMapTable IntPlot s2.png File:GeneMapTable IntPlot s3.png File:GeneMapTable IntPlot s4.png


Mapped gene position (S2)

File:OverlapMark s2.png


Mapped gene count in all 4 tiles

File:GeneMapTable All AgiPPgDNACount.png


Compare to gDNACount of Agi26k0gap Padlock probe

File:GeneMapTable All AgiPPgDNACount Plot.png