Hosuk:LabNotes/2014-9-5
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PGP1F 2nd rolonyar20140414 – Decode again with 63x
- 63x, zoom=1, 2x2 tile (each tile index : s0~s3, --> converted to s1~s4 for analysis)
- Z=0.49um, ~22 images
Result
- Image signal were too saturated… need to use less PMT gain… (this time it was 660, 4% laser)
Merged and each Tile image of BF, 1st rolonies
File:Merged Tile BF.png File:Merged Tile 1stRolony.png
Mapped table and intensity plot
File:GeneMapTable IntPlot s1.png File:GeneMapTable IntPlot s2.png File:GeneMapTable IntPlot s3.png File:GeneMapTable IntPlot s4.png
Mapped gene position (S2)
Mapped gene count in all 4 tiles
File:GeneMapTable All AgiPPgDNACount.png