Noi/NOTES/2014-9-8
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Preparation of WGBS libraries of ctDNA/cfDNA from Illumina (no DNA fragmentation step)
Sample info.
[[1]]
Re-quantification of cfDNA by Qubit dsDNA HS assay
Sample | Conc. By Qubit (ng/ul) | Amount in 20ul (ng) |
NORM1-02 P1 | 1.66 | 33.20 |
NORM1-03 P1 | 0.77 | 15.30 |
COLO2-03 | 1.11 | 22.20 |
- Note that Alan has taken 10ng of each sample for WGBS library prep . I have no time to wait until the library prep done, so I perform experiment in parallel using 5ng of input DNA to make sure that I can continue BSPP capture and get the library ready for Illumina by this Friday.