Noi/NOTES/2014-9-8

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Preparation of WGBS libraries of ctDNA/cfDNA from Illumina (no DNA fragmentation step)

Calendar

Sample info.

[[1]]

Re-quantification of cfDNA by Qubit dsDNA HS assay

Sample Conc. By Qubit (ng/ul) Amount in 20ul (ng)
NORM1-02 P1 1.66 33.20
NORM1-03 P1 0.77 15.30
COLO2-03 1.11 22.20
  • Note that Alan has taken 10ng of each sample for WGBS library prep . I have no time to wait until the library prep done, so I perform experiment in parallel using 5ng of input DNA to make sure that I can continue BSPP capture and get the library ready for Illumina by this Friday.