Noi:Samples from Illumina

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Revision as of 19:38, 13 September 2014 by >Noi (Created page with "* I will add previous samples from Xuyu (bisDNA and pre-amplified DNA for BSPP capture) = cfDNA = == 2014-08-12 == Back * Received ~5ul of cfDNA (onl...")
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  • I will add previous samples from Xuyu (bisDNA and pre-amplified DNA for BSPP capture)

cfDNA[edit]

2014-08-12[edit]

Back

  • Received ~5ul of cfDNA (only info. available on the sample tube) from Xuyu. She said the concentration is 16g/ul. I add more TE buffer to bring volume to 20l and re-quantified concentration by Qubit dsDNA HS assay. The concentration is 7.09ng/ul.

2014-08-25[edit]

Back

  • Dr. Zhang received 3 additional cfDNA from Illumina
  • Sample info. from Rigo
Sample Conc. (ng/ul) Volume (ul) Amount (ng)
NORM1-02 P1 5.25 15.00 78.70
NORM1-03 P1 3.25 15.00 48.70
COLO2-03 3.05 15.00 45.80
  • I am not sure how they measure DNA concentration. I will repeat measuring concentration again.
  • To avoid losing too much DNA for re-quantification, I will add more ~7ul of EB buffer to bring the volume to 21ul and use 1ul for Qubit dsDNA quantification.

Re-quantification of cfDNA by Qubit dsDNA HS assay[edit]

Sample Conc. By Qubit (ng/ul) Amount in 20ul (ng)
NORM1-02 P1 1.66 33.20
NORM1-03 P1 0.77 15.30
COLO2-03 1.11 22.20
  • I guess that the concentration of cfDNA from Rigo is by Nanodrop. Those numbers seemed to be over-estimated since the total amount by Qubit is much lower than that I got from Illumina.