Sam:LabNotes/Microbione/2009-2-3

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Revision as of 06:24, 9 February 2009 by >Sam Chiang (New page: ='''Primer design for human 18S and Bacteria 16S'''= ==Objective== Besides the realtime monitoring, #Design 18S primers for positive control / validation purpose of a successful MDA rea...)
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Primer design for human 18S and Bacteria 16S

Objective

Besides the realtime monitoring,

  1. Design 18S primers for positive control / validation purpose of a successful MDA reaction using human genome template.
  2. Design 16S primers for positive control / validation purpose of a successful MDA reaction using bacteria genome template.
  3. These primers could also be used to detect the contamination of exogenus gDNA.


Human S18 primer

  1. Collect the human S18 cDNA sequence
  2. Mask the inconsistat nucleotide
  3. Paste the