Daniel:Notebook/RNAFISH/2014-10-13

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Revision as of 15:08, 13 October 2014 by >Djacobse (Created page with "=ABP Trial Run (Started 10/10/2014)= Back to Calendar ==Post Hybridization== #2xSSPE/0.6% Triton X-100 ...")
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ABP Trial Run (Started 10/10/2014)[edit]

Back to Calendar

Post Hybridization[edit]

  1. 2xSSPE/0.6% Triton X-100 2x 45mins. At room temp
  2. 1xHSB, 3x 30 mins at hybridization temperature
  3. Add 20 uL DAPI with Slow Fade Gold, cover with coverslip, seal with nail polish
  • High Stringency Buffer:
    • H2O 90ml
    • 0.02M Na4P2O7 10ml
    • 0.2M NaH2PO4 1.5ml