Hosuk:LabNotes/2014-10-16
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Human Brain Tissue : fixing test[edit]
- Try Human Brain Tissue fixed with 4% Formaldehyde at 4C for 12hr.
- Continued from 2014-10-15
Procedure[edit]
- 10/16
- Wash with PBS once
- Prepare 8uL of BS(PEG)9 in 392uL PBS, add 400uL BS(PEG)9 mix, incubate for 1hr at RT
- Wash with PBS once, add Tris pH 8.0, incubate for 30min
- Wash with PBS twice, add 2mL PBS store at 4C
- Imaging 10/16 or 10/17