Hosuk:LabNotes/2014-10-16

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Revision as of 19:47, 16 October 2014 by >Hosuki78
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Human Brain Tissue : fixing test[edit]

  • Try Human Brain Tissue fixed with 4% Formaldehyde at 4C for 12hr.
  • Continued from 2014-10-15


Procedure[edit]

  • 10/16
  • Wash with PBS once
  • Prepare 8uL of BS(PEG)9 in 392uL PBS, add 400uL BS(PEG)9 mix, incubate for 1hr at RT
  • Wash with PBS once, add Tris pH 8.0, incubate for 30min
  • Wash with PBS twice, add 2mL PBS store at 4C


  • Imaging 10/16 or 10/17