Daniel:Notebook/HiResChrPaint/2014-11-5

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Revision as of 17:34, 5 November 2014 by >Djacobse (Created page with "=FISH (Started 11/4/2014) Back to Calendar ==Hybridization== #Remove coverslip carefully and w...")
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=FISH (Started 11/4/2014)

Back to Calendar

Hybridization

  1. Remove coverslip carefully and wash slides in pre-warmed jar containing 2X SSCT at 60C for 15 minutes
  2. Incubate slides in 2X SSCT at room temperature for 10 minutes
  3. Incubate slides in 0.2X SSC at room temperature for 10 minutes
  4. Tap slides dry on papertowels; do not let the surface containing the cells touch the paper towels
  5. Add 15 uL mounting media such as SlowFadeGold with DAPI to a 22x30 #1.5 coverslip
  6. Invert slides onto coverslips with mounting media
  7. Seal each slide using nail polish
  8. Allow at least 30 minutes for nail polish to dry
  9. Slides are quite stable for several months at 4C