Noi/NOTES/2014-11-25
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Comparison of the efficiency between TruSeq adaptor and an unmethylated customized Y-adaptor with UMI
- Dr. Zhang wanted to compare the efficiency of standard adaptor and a customized Y-adaptor with UMI for generating sequencing libraries using KAPA Hyper Prep kit. In this experiment, I used TruSeq adaptor to represent standard adaptor.
- I received top and bottom oligos to generated Y-adaptor oligo --> final conc. 15uM to be equivalent to the concentration of TruSeq adaptor
Conditions