Blue:RNA-Seq Experiments:12012014

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Revision as of 02:10, 2 December 2014 by >B1lake (→‎LiuKai1-4 Plates: NexteraXT Library Preparation)
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LiuKai1-4 Plates: NexteraXT Library Preparation[edit]

  • Repeated Nextera on Normalized Samples prepared by Derek (note: First attempt at Nextera showed smears above 800bp, indicating bad stock of transposase - repeated with fresh stock)
  • Gel purified PCR products twice with 0.8x beads and resuspended in 10ul for each library:
    • LiuKai_1: Nu-iPSC early stage cells
    • LiuKai_2: Nu-iPSC late stage cellss
    • LiuKai_3: Normal iPSC early stage cells
    • LiuKai_4: Normal iPSC late stage cells

Note: The subset of cDNA samples chosen for further processing was decided by Liu Kai

Ran 1ul of each library on gel:

File:12012014 LiuKai Miseq.jpg