Daniel:Notebook/RNAFISH/2014-12-10

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Revision as of 19:35, 10 December 2014 by >Djacobse (Created page with "=Cell Line RNA FISH (Started 12/09/2014)= Back to Calendar ==Post Hybridization== '''Wash Version 2''' #2...")
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Cell Line RNA FISH (Started 12/09/2014)[edit]

Back to Calendar

Post Hybridization[edit]

Wash Version 2

  1. 2x 2xSSC 45 mins room temp
  2. 1x 0.2x SSC 45 mins hybridization temp
  3. Add 20 uL DAPI with Slow Fade Gold, cover with coverslip, seal with nail polish