Dinh:OxBS Protocol

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Revision as of 02:06, 19 December 2014 by >Dinh (Created page with "= OxBS protocol = * Cambridge Epigenetix (CEGX) TrueMethyl kit: although each reaction costs > $100, they can have maximum input of only about 1 ug per reaction provided in th...")
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OxBS protocol

  • Cambridge Epigenetix (CEGX) TrueMethyl kit: although each reaction costs > $100, they can have maximum input of only about 1 ug per reaction provided in the kit. To get enough input for BSPP, we need to use at least 2 reactions from the kit. After working with the kit once with Tiffany, I decided that it would be best to invest in purchasing individual components of the TrueMethyl kit & then making other components ourselves. Since the oxidant reagent is simply a solution of KRuO4 and NaOH we can make this solution & always use a control DNA to quantify the efficiency of conversion.
  • References & notes:

Preparation of the sequencing and digestion spike-in controls

  • The 100 bp control DNA is provided in the TrueMethyl kit.