Noi:Noi' Reagents
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List of reagent in -20C, -80C, and 4C (December 2014)
Nimblegen SeqCap (UMRv1)
- SeqCap Epi probe pool (Design name: 140923_HG19_UMR_v1_Epi_H) was aliquot 4.5ul in each 0.2mL PCR tube as described in 2014-10-29
- There are total 11 tubes left in a small original plastic box that put the cupboard box in -20C freezer labeled Library Prep Kit Noi Zhanglab as shown in picture above.
WGBS libraries of normal and cancer patients
- Please note that there are two batches of WGBS libraries of ctDNA from cancer patients. The first batch I generated using the protocol similar to RRBS but skipped DNA digestion and used 1ng of ctDNA as starting materials as described in XXXX. This batch of WGBS libraries was used for the first Nimblegen SeqCap hybridization capture experiment on XXXX, I have them all (24 libraries in PCR rack that contain in Noi Losen Stuffs 2 at -20C as shown I'n picture above.
- I also have WGBS of cfDNA from normal control generated on XXXX in the same box. However, I have performed re-amplification of these normal samples and stored in different location that will be describe below. I may not suggested to use all WGBS libraries in Noi Losen Stuffs 2 box.
- PLEASE USE WGBS libraries I put in PCR rack with the cover at-20C.
- There are 24 WGBS of normal control re-amplified on XXXX, However, these WGBS are generated by the method similar to RRBS without DNA digestion or any fragmentation. I did not generate more WGBS libraries of normal control using KAPA Hyper Prep Kit as they showed good result either in BSPP capture and SeqCap hybridization capture. Dr. Zhang suggested to generated more only for those cancer samples which have poor results.
- The second batch of WGBS from ctDNA of cancer patients were generated using KAPA Hyper Prep Kit with more input of starting material (in the range of 3-20ng). They were generated on XXXX and should be located in the PCR rack with cover (on the left of image below.