Noi/NOTES/2014-11-30

From ZhangLabWiki
Revision as of 10:41, 12 January 2015 by >Noi (Created page with "= Preparation of WGBS of cfDNA from plasma of cancer patients using KAPA Hyper Prep kit = '''Back to calendar''' * As I mentioned before in previ...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Preparation of WGBS of cfDNA from plasma of cancer patients using KAPA Hyper Prep kit

Back to calendar

  • As I mentioned before in previous experiment that the first batch of WGBS libraries from cfDNA of normal and cancer patients were generated by RRBS method without DNA fragmentation. This might lead to the incomplete of libraries as RRBS protocol uses only Klenow fragment, eco- but lack T4 DNA Pol and T4 PNK enzyme. Dr. Zhang suggested to generated WGBS libraries of cfDNA from cancer patients using KAPA Hyper Prep Kit as I did test experiment on 2014-10-24.
  • I also increased DNA input as much as possible for each sample to be able to capture all individual unique molecule from cancer cells in the DNA pool of cfDNA.