Noi/NOTES/2014-12-16
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LMS (MONOD V5A) BSPP capture on WGBS libraries prepared from cfDNA of normal control and cancer patient
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Continued from 2014-12-05
- Conditions:
- Probe set: MONOD V5 set A (LMS)
- Probe:target ratio: 150:1 for LMS probe set. Please note that as I used very high amount of WGBS libraries for BSPP capture, I reduced probe to target ratio from 200:1 to 150:1. The main reason is I do not have enough LMS BSPP to capture 50 sample with 600ng DNA each.
- Sample: WGBS libraries prepared by KAPA Hyper Prep Kit (2014-12-05) amount 400ng
Probe calculation (Example)
Probe set Probe size Probe:target ratio LMS 73,574 150:1