Arichard:Reagents/hls

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Hypotonic lysis solution (HLS)[edit]

10X stock (Crawford et al. PNAS 2004):

  • 100 mM Tris HCl, pH 7.5
  • 100 mM NaCl
  • 30 mM MgCl2
  • 1% Igepal (Nonidet P-40)

0.22 um filter and UV treat

Can be used at 1X with or without 0.2 Au/ml (4.4 mg/ml) QIAGEN protease (45 mAu/mg)

Brandon: Use without protQ, <5 min at RT, then add 1:1 volume of 8 M Guanidine HCl followed by 1.8:1 Ampure XP