Hosuk:LabNotes/2015-1-25

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Revision as of 21:55, 25 January 2015 by >Hosuki78 (Created page with "*LabNote ===CA12k_Nov2014 : New Padlock Probes=== ====Concentration==== *CA12k_Nov2014 that Matt has produced **V4 primers - CA12k_Nov2014_Contig **V7 p...")
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CA12k_Nov2014 : New Padlock Probes

Concentration

  • CA12k_Nov2014 that Matt has produced
    • V4 primers - CA12k_Nov2014_Contig
    • V7 primers - CA12k_Nov2014_Const


  • 2015-01-09
    • V4 : 10.7 ng/ul --> 10.7 ng/ul / (150nt*325Da/nt + 79Da) = 219nM (38ul)
    • V7 : 6.2 ng/ul --> 6.2 ng/ul / (150nt*325Da/nt + 79Da) = 127nM (18ul)
    • I’ve used 18uL of V4, and 18uL of V7 for the sample at 1/22
      • --> V4 : 103.7nM, V7 : 127nM in 100uL Ampligase Mix
      • --> 18.67 nM of V4, 22.86 nM of V7 in 100uL mix
      • Let’s fix the final concentration of Padlock probes to 100nM in 100uL Ampligase Mix


  • 2015-01-12
    • V7 : 28 ng/ul --> 28 ng/ul / (150nt*325Da/nt + 79Da) = 573nM (18ul)


  • 2015-01-15
    • V4 : 65.3 ng/ul --> 65.3 ng/ul / (150nt*325Da/nt + 79Da) = 1337nM (38ul)
    • V7 : 39.2 ng/ul --> 39.2 ng/ul / (150nt*325Da/nt + 79Da) = 803nM (18ul)




Genes for New Padlock Probes and Jay’s FISSEQ data