Hosuk:LabNotes/2015-1-25
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CA12k_Nov2014 : New Padlock Probes
Concentration
- CA12k_Nov2014 that Matt has produced
- V4 primers - CA12k_Nov2014_Contig
- V7 primers - CA12k_Nov2014_Const
- 2015-01-09
- V4 : 10.7 ng/ul --> 10.7 ng/ul / (150nt*325Da/nt + 79Da) = 219nM (38ul)
- V7 : 6.2 ng/ul --> 6.2 ng/ul / (150nt*325Da/nt + 79Da) = 127nM (18ul)
- I’ve used 18uL of V4, and 18uL of V7 for the sample at 1/22
- --> V4 : 103.7nM, V7 : 127nM in 100uL Ampligase Mix
- --> 18.67 nM of V4, 22.86 nM of V7 in 100uL mix
- Let’s fix the final concentration of Padlock probes to 100nM in 100uL Ampligase Mix
- 2015-01-12
- V7 : 28 ng/ul --> 28 ng/ul / (150nt*325Da/nt + 79Da) = 573nM (18ul)
- 2015-01-15
- V4 : 65.3 ng/ul --> 65.3 ng/ul / (150nt*325Da/nt + 79Da) = 1337nM (38ul)
- V7 : 39.2 ng/ul --> 39.2 ng/ul / (150nt*325Da/nt + 79Da) = 803nM (18ul)