Daniel:Notebook/RNAFISH/2015-1-29

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Revision as of 18:55, 29 January 2015 by >Djacobse (Created page with "=Oligo Set Preparations= Back to Calendar These are the oligos that I ordered on 08/04/2014. Now that I am...")
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Oligo Set Preparations

Back to Calendar

These are the oligos that I ordered on 08/04/2014. Now that I am ready to do the experiment, I need to prep them. First thing is to resuspend the oligos. By order, each oligo has 15 nmol.

  1. Resuspend each oligo in 15 uL nfH2O (1 mM)
  2. Take 2 uL from each oligo well and combine them in a single 1.5 mL tube (total concentration 1 mM)
    1. Store the remaining oligos at -20C
  3. Add 384 uL nfH2O, diluting 5:1, for final concentration of 200 uM
  4. Check concentration in nanodrop at 1:1, 5:1, and 10:1 dilutions for accuracy