Arichard:Protocols/tn5059

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Revision as of 19:24, 25 February 2015 by >Andrew (Created page with "==Tn5059 protocol (Supermutant) 2/25/2015== * Tn5059 was supplied at 25X, with transposons A and B in separate tubes. We are assuming that a 50/50 mix of A/B is 25X. We have ...")
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Tn5059 protocol (Supermutant) 2/25/2015

  • Tn5059 was supplied at 25X, with transposons A and B in separate tubes. We are assuming that a 50/50 mix of A/B is 25X. We have both control transposons and T7 promoter transposons for Brandon's IVT project.
  • Follow the latest Nextera DNA sample prep user guide for tagmentation conditions and PCR. To avoid purification after tagmentation, use Qiagen Protease to inactivate the Tn5.


  1. 5 ul Tagmentation
    1. 1-3 ul samples
    2. 1 ul 5X Tn5 buffer
    3. 1 ul 5X