Arichard:Protocols/tn5059
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Tn5059 protocol (Supermutant) 2/25/2015
- Tn5059 was supplied at 25X, with transposons A and B in separate tubes. We are assuming that a 50/50 mix of A/B is 25X. We have both control transposons and T7 promoter transposons for Brandon's IVT project.
- Follow the latest Nextera DNA sample prep user guide for tagmentation conditions and PCR. To avoid purification after tagmentation, use Qiagen Protease to inactivate the Tn5.
- 5 ul Tagmentation
- 1-3 ul samples
- 1 ul 5X Tn5 buffer
- 1 ul 5X