Daniel:Notebook/RNAFISH/2015-2-26
Jump to navigation
Jump to search
Linnarsson Protocol (Started 2/24/2015)[edit]
Post-Hybridization[edit]
- Warm up wash buffer and wash buffer with DAPI to 37C
- Rinse with wash buffer
- Add fresh was buffer and incubate for 30 min at 37C
- Replace with fresh wash buffer with DAPI and incubate for 30 min at 37C
- Wash 2 times with warm 2X SSC
- Mount the slide