Daniel:Notebook/RNAFISH/2015-2-26

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Revision as of 16:11, 26 February 2015 by >Djacobse (Created page with "=Linnarsson Protocol (Started 2/24/2015)= Back to Calendar ==Post-Hybridization== #Warm up wash buffer and...")
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Linnarsson Protocol (Started 2/24/2015)[edit]

Back to Calendar

Post-Hybridization[edit]

  1. Warm up wash buffer and wash buffer with DAPI to 37C
  2. Rinse with wash buffer
  3. Add fresh was buffer and incubate for 30 min at 37C
  4. Replace with fresh wash buffer with DAPI and incubate for 30 min at 37C
  5. Wash 2 times with warm 2X SSC
  6. Mount the slide