Andrew:Notebook/C1 InTube 31nL MDA 150320

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Revision as of 21:00, 27 March 2015 by >Andrew (Created page with "==In tube validation for 31 nL MDA protocol 3/20/2015== Motivation *The C1 Open App IFC contains 5 chambers, plus the capture site *This places a constraint on protocol desig...")
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In tube validation for 31 nL MDA protocol 3/20/2015

Motivation

  • The C1 Open App IFC contains 5 chambers, plus the capture site
  • This places a constraint on protocol design, which must be validated in-tube

Objective

  • Test a number of conditions with a scaled-up volume and select the best condition based on sequencing results

Plan

  • I will start with 1 ul and scale all other volumes proportionately.

The scaling factor is 222 = 1 ul/4.5 nl, which is the actual starting volume of the C1 IFC.

  • I will therefore use ~220 nuclei per condition

Because the C1 IFC adds reagents succesively, each step requires a concentration adjustment.