Andrew:Notebook/C1 InTube 31nL MDA 150320
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In tube validation for 31 nL MDA protocol 3/20/2015
Motivation
- The C1 Open App IFC contains 5 chambers, plus the capture site
- This places a constraint on protocol design, which must be validated in-tube
Objective
- Test a number of conditions with a scaled-up volume and select the best condition based on sequencing results
Plan
- I will start with 1 ul and scale all other volumes proportionately.
The scaling factor is 222 = 1 ul/4.5 nl, which is the actual starting volume of the C1 IFC.
- I will therefore use ~220 nuclei per condition
Because the C1 IFC adds reagents succesively, each step requires a concentration adjustment.