Daniel:Notebook/RNAFACS/2015-4-8

From ZhangLabWiki
Revision as of 21:21, 8 April 2015 by >Djacobse (Created page with "=In Tube FISH Test= Back to Calendar Time to begin. The Chun lab should be providing me nuclei later today, and I want to begin ASAP. ==Buffer ...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

In Tube FISH Test

Back to Calendar

Time to begin. The Chun lab should be providing me nuclei later today, and I want to begin ASAP.

Buffer Prep

Reagent Stock Concentration Final Concentration Dilution Volume in 10 mL
SSC Buffer 20X 2X 1:10 1 mL
Ammonium Sulfate NA, powder 2.1 M NA 2.775g
EDTA 0.5 M 10 mM 1:50 200 uL
E. coli tRNA 20 mg/mL 1 mg/mL 1:20 500 uL
BSA 50 mg/mL 500 ug/mL 1:100 100 uL
Formamide 100% 25% 1:04 2.5 mL
nfH2O - - - Fill to 10mL (~5 mL)