Daniel:Notebook/RNAFACS/2015-4-8
Jump to navigation
Jump to search
In Tube FISH Test
Time to begin. The Chun lab should be providing me nuclei later today, and I want to begin ASAP.
Buffer Prep
Reagent | Stock Concentration | Final Concentration | Dilution | Volume in 10 mL |
SSC Buffer | 20X | 2X | 1:10 | 1 mL |
Ammonium Sulfate | NA, powder | 2.1 M | NA | 2.775g |
EDTA | 0.5 M | 10 mM | 1:50 | 200 uL |
E. coli tRNA | 20 mg/mL | 1 mg/mL | 1:20 | 500 uL |
BSA | 50 mg/mL | 500 ug/mL | 1:100 | 100 uL |
Formamide | 100% | 25% | 1:04 | 2.5 mL |
nfH2O | - | - | - | Fill to 10mL (~5 mL) |