Daniel:Notebook/RNAFACS/2015-4-11

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In Tube FISH Test (Started 4/10/2015)

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Dye Labeling

  1. Centrifuge at 4 C for 30 minutes (14000 rpm)
  2. Remove supernatant and add 750 uL chilled 75% EtOH
  3. Centrifuge at minutes at 4 C
  4. Dry the pellet in the hood
  5. Resuspend pellet in 12 uL TE

Hybridization

  1. Resuspend cells in RPHB with labeled probes (0.5-1ng/uL)
  2. Incubate overnight at 30C