Daniel:Notebook/RNAFACS/2015-4-11
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In Tube FISH Test (Started 4/10/2015)
Dye Labeling
- Centrifuge at 4 C for 30 minutes (14000 rpm)
- Remove supernatant and add 750 uL chilled 75% EtOH
- Centrifuge at minutes at 4 C
- Dry the pellet in the hood
- Resuspend pellet in 12 uL TE
Hybridization
- Resuspend cells in RPHB with labeled probes (0.5-1ng/uL)
- Incubate overnight at 30C