Sam:LabNotes/Microbione/2009-3-3/exp1

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Revision as of 17:45, 5 March 2009 by >Sam Chiang (→‎Slide washing)
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Slide washing (Tween 20/PBS washing)

  1. Add 20 uL of Tween 20 to 180 uL of H20 (10% dilution).
  2. Add 19.2 mL of PBS to the solution for a final dilution of .1% Tween 20.
  3. Shake until a soapy consistency is observed
  4. Gently spray 5 ml of the solution over the microarrays
  5. Wipe carefully with a kimwipe and allow to dry

Beads loading

Microscope image

  • Beads in 1/100 dilution, objective 20X (left); Beads in 1/100 dilution, objective 10X (right)
  File:Bead-3-4-09 1-100 2.bmp    File:Bead-3-4-09 1-100.bmp 


  • Beads in 1/200 dilution, objective 20X (left); Beads in 1/200 dilution, objective 20X (right)
  File:Bead-3-4-09 1-200 2.bmp    File:Bead-3-4-09 1-200.bmp