293T Cell Passaging Protocol
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- Add 1% anti-anti (in fridge) and 10% Fetal Bovine Serum (FBS) to DMEM media. Warm media in 37 C water bath.
- Suck out media from flask.
- Add 4 mL Trypsin to flask. Incubate at 37 C incubator for 5 minutes
- Add 6 mL media to quench Trypsin
- From the flask, add 2.5 mL to each 15 cm dish and 1 mL to each flask.
- Add media up to 25 mL per 15 cm dish and 15 mL per flask. Gently shake back and forth and side to side (never in a circle).