Gibson Assembly Protocol
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Revision as of 22:26, 6 May 2015 by
>YanWu
(Created page with "*At least 10:1 insert:LGP ratio by moles *50 uL Gibson Mix *Ultrapure water up to 100 uL total reaction volume *Incubate for 1 hr at 50 C in thermocycler")
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At least 10:1 insert:LGP ratio by moles
50 uL Gibson Mix
Ultrapure water up to 100 uL total reaction volume
Incubate for 1 hr at 50 C in thermocycler
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