Ns126:Calendar/NOTES/2015-6-5

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In this analysis, 15 pairs solid tissue and plasma dataset were collected. The regions with higher MHL in solid tissues than in plasma were considered as tissue dominant MHL region and likewise higher MHL in plasma than in solid tissues were considered as plasma dominant MHL regions. As our previous assumption, cell-free DNA typically come from apoptotic cells, and are in small fragments (Chan et al. 2004) while whole blood DNA typically have larger sizes (at least kilobases) even after DNA extraction. Therefore, we hypothesize that plasma dominant MHL region is longer than tissue domiant MHL regions. The result is as the following, the result show that the length of the length of the plasma domiant MHL are signficant longer than tissue domiant MHL in all of 15 dataset.


Table. Significant difference of the length MHL in tissues and plasmas

length.of.MHL.in.tissue length.of.MHL.in.plasma foldchange delta(bp) P-value
6-P-1 138 164 1.19 26 1.43E-25
6-P-2 149 154 1.03 5 0.043421757
6-P-3 146 159 1.09 13 1.69E-09
6-P-4 136 157 1.15 21 3.10E-19
6-P-5 135 166 1.23 31 6.94E-32
7-P-1 138 159 1.15 21 3.36E-23
7-P-2 137 169 1.23 32 5.64E-32
7-P-3 137 167 1.22 30 1.74E-31
7-P-4 139 158 1.14 19 1.63E-18
7-P-5 132 177 1.34 45 5.71E-66
PC-P-1 131 171 1.31 40 1.86E-45
PC-P-2 133 161 1.21 28 6.73E-37
PC-P-4 127 167 1.31 40 1.61E-62
PC-P-6 132 176 1.33 44 3.05E-66
PC-P-7 128 171 1.34 43 3.75E-63