Matt:LabNotes/2015-7-9

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DARTFISH Imaging Neurons

Universal Cy3 Dye-Probe check

  • Add 75C pre-heated 0.5uM FISGA_Adpt (Cy3) (1ul 100uM FISGA_Adpt + 199ul 2X SSC + 30% formamide)
  • Wash with 2X SSC twice and add 2X SSC for imaging