Brandon:LabNotes/Project1/2015-8-3

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Revision as of 02:27, 4 August 2015 by >Bsos (Created page with "==Update of THS-seq protocol sequences to use [http://genome-tech.ucsd.edu/LabNotes/index.php/Brandon:Protocols/Nextera_XT_V2_sequences_and_outline Nextera XT V2 sequences]== ...")
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Update of THS-seq protocol sequences to use Nextera XT V2 sequences



  • Called illumina and they said it is either sequencer not being cleaned right, or concentrations are off, or sequences in library generation protocol are not compatible. Considering currently old nextera sequences are being used from 5 years ago, and all current systems illumina sequencing systems do not use them, then maybe that is the problem.


  • Thus will redesign THS-seq using Nextera XT V2 sequencing/index/flowcell/amplification primers.