Sam:LabNotes/Microbiome/2009-3-10
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'Perform MDA on human gDNA, diluted whole cells
Sample & Material
- Cell: GM18506 cell line, harvested, washed by UV-treated PBS and coundted to 200,000 cell/mL (200 cell/uL)
- Dilute to 10 cell/uL, 5 cell/uL, 0.5 cell/uL using UV-treated PBS
Dilute factor original x 1/2 x 1/10 x 1/2 x 1/10 conc.(cell/uL) 200/uL 100/uL 10/uL 5/uL 0.5/uL Transfering(uL) 50 10 50 10 UVed-PBS(uL) 50 90 50 90
- Pos. control: human gDNA diluted to 3 ng/uL
- Measure the original (commercial human gDNA) using Nanodrop -> 309ng/uL
Dilute factor original x 1/10 x 1/10 x 1/10 x 1/10 conc. 309ng/uL 3ng/uL 300pg/uL 30pg/uL 3pg/uL Transfering(uL) 10 10 10 10 UVed-PBS(uL) 90 90 90 90