Sam:LabNotes/Microbiome/2009-3-10

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Revision as of 17:06, 11 March 2009 by >Sam Chiang (New page: ='Perform MDA on human gDNA, diluted whole cells'''= ==Sample & Material== *Cell: GM18506 cell line, harvested, washed by UV-treated PBS and coundted to 200,000 cell/mL (200 cell/uL) **D...)
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'Perform MDA on human gDNA, diluted whole cells

Sample & Material

  • Cell: GM18506 cell line, harvested, washed by UV-treated PBS and coundted to 200,000 cell/mL (200 cell/uL)
    • Dilute to 10 cell/uL, 5 cell/uL, 0.5 cell/uL using UV-treated PBS
  Dilute factor     original    x 1/2   x 1/10   x 1/2    x 1/10
  conc.(cell/uL)      200/uL   100/uL    10/uL    5/uL    0.5/uL
  Transfering(uL)           50        10       50      10                     
  UVed-PBS(uL)                   50       90       50      90


  • Pos. control: human gDNA diluted to 3 ng/uL
    • Measure the original (commercial human gDNA) using Nanodrop -> 309ng/uL
  Dilute factor    original      x 1/10       x 1/10     x 1/10    x 1/10
  conc.            309ng/uL      3ng/uL     300pg/uL    30pg/uL    3pg/uL
  Transfering(uL)           10           10          10         10                     
  UVed-PBS(uL)                     90           90         90         90