Dinh/Dinh 2015/NOTES/2015-9-16
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RRBS with input 1.5 ng from plasma DNA
- There are a total of 40 samples to be processed.
- TruSeq adaptors have a total of 24 different barcodes.
Plasma Sample | Sample Concentration (ng/uL) | Volume eluted in EB (uL) | Yield (ng) | Plasma volume (uL) | Est. yield from 1 mL (ng) | Volume for 1.5 ng (uL) | Volume water to make 12 uL (uL) |
7.12 | 0.16 | 30 | 4.68 | 535 | 8.75 | 9.62 | 2.38 |
6.27 | 0.17 | 30 | 5.10 | 55 | 92.73 | 8.82 | 3.18 |
6.20 | 0.18 | 30 | 5.40 | 641 | 8.42 | 8.33 | 3.67 |
6.15 | 0.19 | 30 | 5.82 | 610 | 9.54 | 7.73 | 4.27 |
6.25 | 0.23 | 30 | 6.96 | 150 | 46.40 | 6.47 | 5.53 |
6.11 | 0.25 | 30 | 7.38 | 231 | 31.95 | 6.10 | 5.90 |
7.26 | 0.26 | 30 | 7.92 | 690 | 11.48 | 5.68 | 6.32 |
6.17 | 0.27 | 30 | 7.98 | 480 | 16.63 | 5.64 | 6.36 |
7.29 | 0.28 | 30 | 8.28 | 690 | 12.00 | 5.43 | 6.57 |
7.25 | 0.29 | 30 | 8.76 | 700 | 12.51 | 5.14 | 6.86 |
7.16 | 0.29 | 30 | 8.82 | 630 | 14.00 | 5.10 | 6.90 |
6.12 | 0.31 | 30 | 9.36 | 670 | 13.97 | 4.81 | 7.19 |
6.29 | 0.31 | 30 | 9.42 | 940 | 10.02 | 4.78 | 7.22 |
6.18 | 0.32 | 30 | 9.48 | 425 | 22.31 | 4.75 | 7.25 |
7.13 | 0.32 | 30 | 9.66 | 630 | 15.33 | 4.66 | 7.34 |
7.15 | 0.32 | 30 | 9.66 | 455 | 21.23 | 4.66 | 7.34 |
6.26 | 0.33 | 30 | 9.84 | 550 | 17.89 | 4.57 | 7.43 |
6.24 | 0.37 | 30 | 10.98 | 725 | 15.14 | 4.10 | 7.90 |
7.21 | 0.37 | 30 | 11.04 | 620 | 17.81 | 4.08 | 7.92 |
7.14 | 0.37 | 30 | 11.10 | 600 | 18.50 | 4.05 | 7.95 |
6.21 | 0.38 | 30 | 11.40 | 670 | 17.01 | 3.95 | 8.05 |
6.16 | 0.39 | 30 | 11.76 | 650 | 18.09 | 3.83 | 8.17 |
6.19 | 0.39 | 30 | 11.82 | 650 | 18.18 | 3.81 | 8.19 |
7.11 | 0.42 | 30 | 12.66 | 595 | 21.28 | 3.55 | 8.45 |
6.30 | 0.43 | 30 | 12.84 | 405 | 31.70 | 3.50 | 8.50 |
7.19 | 0.44 | 30 | 13.20 | 760 | 17.37 | 3.41 | 8.59 |
6.14 | 0.48 | 30 | 14.28 | 585 | 24.41 | 3.15 | 8.85 |
7.27 | 0.55 | 30 | 16.50 | 700 | 23.57 | 2.73 | 9.27 |
7.20 | 0.75 | 30 | 22.38 | 550 | 40.69 | 2.01 | 9.99 |
7.22 | 0.77 | 30 | 23.04 | 385 | 59.84 | 1.95 | 10.05 |
6.23 | 0.88 | 30 | 26.34 | 900 | 29.27 | 1.71 | 10.29 |
ALL | 0.94 | 30 | 28.08 | NA | NA | 1.60 | 10.40 |
7.18 | 0.71 | 40 | 28.24 | 430 | 65.67 | 2.12 | 9.88 |
6.13 | 1.04 | 30 | 31.26 | 690 | 45.30 | 1.44 | 10.56 |
7.28 | 1.06 | 30 | 31.80 | 780 | 40.77 | 1.42 | 10.58 |
7.17 | 1.09 | 30 | 32.58 | 355 | 91.77 | 1.38 | 10.62 |
6.22 | 1.29 | 30 | 38.64 | 460 | 84.00 | 1.16 | 10.84 |
7.23 | 1.20 | 40 | 48.00 | 540 | 88.89 | 1.25 | 10.75 |
7.30 | 2.32 | 30 | 69.60 | 398 | 174.87 | 0.65 | 11.35 |
6.28 | 4.08 | 30 | 122.40 | 940 | 130.21 | 0.37 | 11.63 |
7.24 | 106.00 | 40 | 4240.00 | 685 | 6189.78 | 0.01 | 11.99 |