Brandon:LabNotes/Project1/2015-9-18
combinatorial method sequences detail
i5 universal connector AATGATACGGCGACCACCGAGATCTACAC i5_nXTv2 index primer AATGATACGGCGACCACCGAGATCTACAC[CTCTCTAT]TCGTCGGCAGCGTC i5 barcode + flowcell adaptor AATGATACGGCGACCACCGAGATCTACAC[TATCCTCT]TCGTCGGCAGCGTC AATGATACGGCGACCACCGAGATCTACAC[GTAAGGAG]TCGTCGGCAGCGTC
GCGATCGAGGACGGC [AGATGTGTATAAGAGACAG] read1 TCGTCGGCAGCGTC{TCCACGC}[TATAGCCT]{GCGATCGAGGACGGC}[AGATGTGTATAAGAGACAG] i5_transposon + bc TCGTCGGCAGCGTC{TCCACGC}[ATAGAGGC]{GCGATCGAGGACGGC}[AGATGTGTATAAGAGACAG]
i7 universal connector
CACCGTCTCCGCCTC [AGATGTGTATAAGAGACAG] read 2
GTCTCGTGGGCTCGG{CTGTCCCTGTCC}[CGAGTAAT]{CACCGTCTCCGCCTC}[AGATGTGTATAAGAGACAG] GTCTCGTGGGCTCGG{CTGTCCCTGTCC}[TCTCCGGA]{CACCGTCTCCGCCTC}[AGATGTGTATAAGAGACAG]
GGCTCGGGTGCTCTG[ATTCCGCT]TAGAGCATACGGCAGAAGACGAAC i7 bc+ flowcell adaptor GGCTCGGGTGCTCTG[GCTCCGAC]TAGAGCATACGGCAGAAGACGAAC
GACAGAGAATATGTGTAGA{CTCCGCCTCTGCCAC} <- read2 GACAGAGAATATGTGTAGA{CTCCGCCTCTGCCAC}[TAATGAGC]{CCTGTCCCTGTC}GGCTCGGGTGCTCTG i7_transposon = bc GACAGAGAATATGTGTAGA{CTCCGCCTCTGCCAC}[AGGCCTCT]{CCTGTCCCTGTC}GGCTCGGGTGCTCTG CTGTCTCTTATACACATCT{GAGGCGGAGACGGTG} -> index 1