Brandon:LabNotes/Project1/2015-9-18

From ZhangLabWiki
Revision as of 02:18, 19 September 2015 by >Bsos (Created page with "==combinatorial method sequences detail == i5 universal connector AATGATACGGCGACCACCGAGATCTACAC i5_nXTv2 index primer AATGATACGGCGACCACCGAGATCTACAC[CTCTCTAT]TCGTCGGCAGCGTC ...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

combinatorial method sequences detail

i5 universal connector AATGATACGGCGACCACCGAGATCTACAC i5_nXTv2 index primer AATGATACGGCGACCACCGAGATCTACAC[CTCTCTAT]TCGTCGGCAGCGTC i5 barcode + flowcell adaptor AATGATACGGCGACCACCGAGATCTACAC[TATCCTCT]TCGTCGGCAGCGTC AATGATACGGCGACCACCGAGATCTACAC[GTAAGGAG]TCGTCGGCAGCGTC

                                                                        GCGATCGAGGACGGC [AGATGTGTATAAGAGACAG]  read1
                                      TCGTCGGCAGCGTC{TCCACGC}[TATAGCCT]{GCGATCGAGGACGGC}[AGATGTGTATAAGAGACAG]  i5_transposon + bc
                                      TCGTCGGCAGCGTC{TCCACGC}[ATAGAGGC]{GCGATCGAGGACGGC}[AGATGTGTATAAGAGACAG]  
                                                                     

i7 universal connector

                                       CACCGTCTCCGCCTC [AGATGTGTATAAGAGACAG] read 2

GTCTCGTGGGCTCGG{CTGTCCCTGTCC}[CGAGTAAT]{CACCGTCTCCGCCTC}[AGATGTGTATAAGAGACAG] GTCTCGTGGGCTCGG{CTGTCCCTGTCC}[TCTCCGGA]{CACCGTCTCCGCCTC}[AGATGTGTATAAGAGACAG]


                                                           GGCTCGGGTGCTCTG[ATTCCGCT]TAGAGCATACGGCAGAAGACGAAC i7 bc+ flowcell adaptor
                                                           GGCTCGGGTGCTCTG[GCTCCGAC]TAGAGCATACGGCAGAAGACGAAC

GACAGAGAATATGTGTAGA{CTCCGCCTCTGCCAC} <- read2 GACAGAGAATATGTGTAGA{CTCCGCCTCTGCCAC}[TAATGAGC]{CCTGTCCCTGTC}GGCTCGGGTGCTCTG i7_transposon = bc GACAGAGAATATGTGTAGA{CTCCGCCTCTGCCAC}[AGGCCTCT]{CCTGTCCCTGTC}GGCTCGGGTGCTCTG CTGTCTCTTATACACATCT{GAGGCGGAGACGGTG} -> index 1