Kun:LabNotes/ASE/2007-11-26

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Revision as of 00:55, 27 November 2007 by KunZhang (talk | contribs) (New page: ==Exp.1 PAGE purification and quantification of CES22k solexa libraries== I purified 12 PCR amplicons from the exonic SNP capturing experiment did on 11-20-2007. 40ul per amplicon into ea...)
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Exp.1 PAGE purification and quantification of CES22k solexa libraries

I purified 12 PCR amplicons from the exonic SNP capturing experiment did on 11-20-2007. 40ul per amplicon into each lane of the 5-well gels.

 File:2007-11-24-CES22k-exonic-capture-PAGE-purification.png
 File:2007-11-26-CES22k-sollib-quant.png
 Quantification:
 1. NA20431   4.4ng/ul     110nM
 2. GM20431   4.2ng/ul     100nM
 3. GC1F      3.9ng/ul      94nM
 4. GC1EP     3.4ng/ul      80nM
 >------shipped to VCU on 11-26-2007-----<
 5. Hues6g    1.2ng/ul     28nM
 6. Hues6c    2.8ng/ul     68nM
 7. Hybrid 1g 4.5ng/ul    110nM
 8. Hybrid 1c 4.6ng/ul    110nM
 9. Hybrid 2g 3.7ng/ul     87nM
 10.Hybrid 2c 3.2ng/ul     75nM 
 11.BJg       0.34ng/ul     8nM
 12.BJc       3.0ng/ul     71nM