Kun:LabNotes/ASE/2007-11-26
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Exp.1 PAGE purification and quantification of CES22k solexa libraries
I purified 12 PCR amplicons from the exonic SNP capturing experiment did on 11-20-2007. 40ul per amplicon into each lane of the 5-well gels.
File:2007-11-24-CES22k-exonic-capture-PAGE-purification.png
File:2007-11-26-CES22k-sollib-quant.png
Quantification: 1. NA20431 4.4ng/ul 110nM 2. GM20431 4.2ng/ul 100nM 3. GC1F 3.9ng/ul 94nM 4. GC1EP 3.4ng/ul 80nM >------shipped to VCU on 11-26-2007-----< 5. Hues6g 1.2ng/ul 28nM 6. Hues6c 2.8ng/ul 68nM 7. Hybrid 1g 4.5ng/ul 110nM 8. Hybrid 1c 4.6ng/ul 110nM 9. Hybrid 2g 3.7ng/ul 87nM 10.Hybrid 2c 3.2ng/ul 75nM 11.BJg 0.34ng/ul 8nM 12.BJc 3.0ng/ul 71nM