Ns126:Calendar/NOTES/2016-2-18

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Revision as of 10:24, 26 February 2016 by >Shicheng (→‎Result)
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Methylation Haplotype Block Plot

purpose

plot MHB heatmap block

Method

  • Bam2haploinfo from GWBS dataset
  • Merge All the haploinfo files
cd /home/shg047/oasis/monod/haplo/
cat /home/shg047/oasis/monod/haplo/n37/*hapInfo.txt >> hapinfo.txt
cat /home/shg047/oasis/monod/haplo/wbc/*hapInfo.txt >> hapinfo.txt
cat /home/shg047/oasis/monod/haplo/hesc/*hapInfo.txt >> hapinfo.txt
cat /home/shg047/oasis/monod/haplo/tumor_wgbs/*hapInfo.txt >> hapinfo.txt
cat /home/shg047/oasis/monod/haplo/salk/*hapInfo.txt >> hapinfo.txt
  • Haploinfo to R-square matrix by Genomic Region
cd /home/shg047/oasis/monod/haplo/
perl ~/oasis/monod/bin/haploinfo2LDR2.pl  APC chr5:111986595-112228720 < /home/shg047/oasis/monod/haplo/hapinfo.txt
perl ~/oasis/monod/bin/haploinfo2LDR2.pl  ZNF154 chr19:58206356-58222715 < /home/shg047/oasis/monod/haplo/hapinfo.txt
perl ~/oasis/monod/bin/haploinfo2LDR2.pl  SHOX2 chr3:157808200-157829413 < /home/shg047/oasis/monod/haplo/hapinfo.txt
perl ~/oasis/monod/bin/haploinfo2LDR2.pl  DIRAS3 chr1:68511645-68516481 < /home/shg047/oasis/monod/haplo/hapinfo.txt
  • Extent Full R-square matrix by Human Genome CpG sites
perl /home/shg047/oasis/monod/bin/LDR2extent.pl chr5:112039140-112047142 APC.chr5.sqr > APC.full.region.sqr

Result

  • Partial CpG Mode Figure

50*50 50*50

  • Full CpG mode Figure
perl ~/oasis/monod/bin/haploinfo2LDR2.pl  APC.ext chr5:112043054-112197528 < /home/shg047/oasis/monod/haplo/hapinfo.txt
perl /home/shg047/bin/LDR2extent.pl chr5:112043054-112197528 APC.chr5.rsq /home/kunzhang/HsGenome/hg19/HsGenome19.CpG.positions.txt > APC.chr5.rsq.ext
  • R plot script
library("grDevices")
col=colorRampPalette(c("white", "red"))(200)
M <- read.table("APC.chr5.rsq.ext")
dim(M)
M<-data.matrix(M)
M[is.na(M)]<-0
M[lower.tri(M)] <- NA
Name1<-unlist(strsplit(filename,"[.]"))[1]
chr<-unlist(strsplit(filename,"[.]"))[2]
start<-unlist(strsplit(colnames(M)[1],"X"))[2]
end<-unlist(strsplit(colnames(M)[ncol(M)],"X"))[2]
xlab=paste(Name1,"(",chr,":",start,"-",end,")",sep="")
xlab=paste(Name1,"(",chr,":",112043054,"-",112197528,")",sep="")
jpeg("APC.LD.block-3.jpg")
image(M,col = col,xaxt="n",yaxt="n",main=xlab)
dev.off()

50*5030*30

Conclusion