Daniel:Notebook/ComboLock/2016-3-15

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Revision as of 21:32, 15 March 2016 by >Djacobse (→‎VA-044/10% Acrylamide test)
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10% Acrylamide VA-044

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From yesterday's results we decided to increase the acrylamide concentration in the gel solution in order to make up for a lack of formaldehyde/tissue.

VA-044/10% Acrylamide test

For test 2 we used the same setup as test 1, but with the following recipe for the VA-044 gel mix:

Reagent Stock Concentration Final Concentration Amount added
Acrylamide 40% 9% 6.75 mL
Acrylamide:Bis (19:1) 40% (38:2) 0.95:0.05 (%) 0.75 mL
VA-044 ----- 0.25% (m/v) 0.075 g
PBS 10X 1X 3 mL
ddH2O ----- ----- 9.5 mL
Total     30 mL


Run Parameters

  • Line 1: Oil (on ice); X psi; ~2 mL
  • Line 2: Aqueous (VA-044P; on ice); X psi; ~1 mL

Results