Daniel:Notebook/ComboLock/2016-3-16

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Revision as of 17:10, 16 March 2016 by >Djacobse (→‎Protocol)
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Drop Seq Test (Started 03/15/2016)

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After yesterday's results it would appear that we may have polyacrylamide droplets. However, I think the best test is a chemical one, so we'll be following the Macosko protocol for droplet breakage. This protocol uses Perfluoro-1-octanol to break apart droplets. If the droplets did form PA beads, then the octanol shouldn't have an effect. If they didn't the droplets should disappear.

Protocol

  1. Wash 3X with Mineral Oil (no Span)
    1. Remove oil phase with a pipette, and add in ~500 uL mineral oil
    2. Pipette the oil and beads gently to mix; repeat
    3. 1.1 and 1.2