Daniel:Notebook/ComboLock/2016-6-28

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Revision as of 21:24, 27 June 2016 by >Djacobse (Created page with "=Antibody Stage 2 Test (Started yesterday)= Back to Calendar ==Protocol== <ol start="2"> <li>Latch an...")
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Antibody Stage 2 Test (Started yesterday)

Back to Calendar

Protocol

  1. Latch and Padlock Hybridization
    1. Heat inactivate enzyme by incubating for 2 minutes at 94C
  2. Exonuclease Digestion
    1. Prepare exonuclease I/III mix by mixing 2 uL exonuclease I (20 units/μL) and 10 uL exonuclease III (100 units/μL) in 1:1 ratio
    2. Add 2 μL of exonuclease I/III mix to reaction
    3. Mix the reaction by swirling pipette around the well 5 times
    4. Incubate reaction at 37 ºC for 2 hours
    5. Heat inactivate enzyme by incubating at 94C for 5 minutes
  3. qPCR *this is actually PCR since the eppendorf thermocycler was down and the other was in use
    1. Set up reaction according to table