Daniel:Notebook/ComboLock/2016-7-1

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Revision as of 15:41, 1 July 2016 by >Djacobse (Created page with "=Antibody Stage 2 Test 2 (Started 6-28-2016)= Continuing from 6-30 <ol start="6"> <li>Ethanol Precipitation</li> <ol type="A" start="...")
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Antibody Stage 2 Test 2 (Started 6-28-2016)

Continuing from 6-30

  1. Ethanol Precipitation
    1. Centrifuge at 4 C for 25 minutes at 14000 rpm
    2. Remove supernatant and add 750 uL chilled 75% EtOH
    3. Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
    4. Remove supernatant and dry the pellet in the hood
    5. Resuspend pellet in 20 uL volume nfH20
    6. Measure in Nanodrop
    7. Store DNA at 4C

Nanodrop Results

Batch Source ng/uL
Ab1  
Ab2