Daniel:Notebook/ComboLock/2016-7-27

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Revision as of 16:24, 28 July 2016 by >Djacobse (→‎Latch 100 and Dextran Test (Started 7-25-2016)
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Latch 100 and Dextran Test (Started 7-25-2016)[edit]

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Library Prep (from yesterday)[edit]

  1. Ethanol precipitation
    1. Centrifuge at 4 C for 25 minutes at 14000 rpm
    2. Remove supernatant and add 750 uL chilled 75% EtOH
    3. Centrifuge for 15 minutes at minutes at 4 C at 14000 rpm
    4. Remove supernatant and dry the pellet in the hood
    5. Resuspend pellet in 20 uL volume nfH20
    6. Measure in Nanodrop
    7. Combined 15 uL from each sample
    8. Store DNA at 4C

Nanodrop Results[edit]

Source ng/uL
7-26 (Latch100/Dextran) A 31.0
7-26 (Latch100/Dextran) B 19.3
Sample ng/uL uL added final mass (ng)
7-26 Sample A 31 15 465
7-26 Sample B 19.3 15 289.5