Daniel:Notebook/ComboLock/2016-8-22
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Positive Control Amplicon Test (Started Thursday)
Protocol
- Ethanol Precipitation
- Centrifuge at 12000rpm at 4C for 25 minutes
- Remove supernatant and add 750 uL 70% EtOH
- Centrifuge at 12000rpm at 4C for 15 minutes
- Remove supernatant and let dry in PCR hood for 15 min
- Resuspend in 30 uL nfH2O