Daniel:Notebook/ComboLock/2016-9-6
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X3 Dilution Test (8/29/2016)
Protocol
- Ethanol Precipitation
- Pellet by centrifuging for 25 minutes at 12000rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 20 uL nfH2O and measure in nanodrop
- Combined 15 uL from each of the two tubes above
- Stored at 4C in upcoming libraries box
Nanodrop Results
Sample | ng/uL | ||||||||||||
Sample A | 31 | ||||||||||||
Sample B | 19.3
Sequencing Library
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