Matt:LabNotes/2016-9-14

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Revision as of 00:47, 15 September 2016 by >Mzcai (Created page with "==CA12k_Nov2014 V6 (RevComp of V4) Probe Production== * Expansion PCR and first time Production PCR ===Production PCR=== *V6 master mix {| {{tab...")
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CA12k_Nov2014 V6 (RevComp of V4) Probe Production

Production PCR

  • V6 master mix
Components Volume (1X) Volume (50X)
First round amplicon CA12k_Nov14_V6 (10nM) 0.2 10
2X KAPA SYBG MM 50 2500
AP1V6U (100uM) 0.4 20
AP2V6 (100uM) 0.4 20
H2O 49 2450
Total 100 5000