Daniel:Notebook/ComboLock/2016-10-12

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Padlock0301 Test (Started Friday 10-7)

Back to Calendar

Testing the amplification using the forward (AmpF-CL-v2) and primer24 reverse set, as well as some dilutions.

Protocol

Sample Molecule Count Lane Reverse Primer Reverse Primer Conc. (uM) Sample Vol (uL) 2X Kapa SYBR qPCR MM Reverse Primer 100 uM AmpF H2O Total Volume (uL) Master Mix
Sample 0A 10 pmol A1 primer24 100 2 25 0.5 0.5 22 50 MM1
Sample 0B 10 pmol A2 primer24 100 2 25 0.5 0.5 22 50 MM1
Sample 0C 10 pmol A3 primer24 100 2 25 0.5 0.5 22 50 MM1
NTC (rev) 0 A4 primer24 100 0 25 0.5 0.5 24 50 MM1
Sample 0A 10 pmol A1 AmpR6.3-27 100 2 25 0.1 0.1 22.8 50 MM2
Sample 0B 10 pmol A2 AmpR6.3-27 100 2 25 0.1 0.1 22.8 50 MM2
Sample 0C 10 pmol A3 AmpR6.3-27 100 2 25 0.1 0.1 22.8 50 MM2
NTC (rev) 0 A4 AmpR6.3-27 100 0 25 0.1 0.1 24 50 MM2
Sample 0A 10 pmol A1 AmpR6.3-28 10 2 25 0.5 0.5 22 50 MM3
Sample 0B 10 pmol A2 AmpR6.3-28 10 2 25 0.5 0.5 22 50 MM3
Sample 0C 10 pmol A3 AmpR6.3-28 10 2 25 0.5 0.5 22 50 MM3
NTC (rev) 0 A4 AmpR6.3-28 10 0 25 0.5 0.5 22 50 MM3
Sample 0A 10 pmol A1 AmpR6.3-29 10 2 25 0.1 0.1 22.8 50 MM4
Sample 0B 10 pmol A2 AmpR6.3-29 10 2 25 0.1 0.1 22.8 50 MM4
Sample 0C 10 pmol A3 AmpR6.3-29 10 2 25 0.1 0.1 22.8 50 MM4
NTC (rev) 0 A4 AmpR6.3-29 10 0 25 0.1 0.1 24 50 MM4