Daniel:Notebook/ComboLock/2016-10-12
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Padlock0301 Test (Started Friday 10-7)
Testing the amplification using the forward (AmpF-CL-v2) and primer24 reverse set, as well as some dilutions.
Protocol
Sample | Molecule Count | Lane | Reverse Primer | Reverse Primer Conc. (uM) | Sample Vol (uL) | 2X Kapa SYBR qPCR MM | Reverse Primer | 100 uM AmpF | H2O | Total Volume (uL) | Master Mix |
Sample 0A | 10 pmol | A1 | primer24 | 100 | 2 | 25 | 0.5 | 0.5 | 22 | 50 | MM1 |
Sample 0B | 10 pmol | A2 | primer24 | 100 | 2 | 25 | 0.5 | 0.5 | 22 | 50 | MM1 |
Sample 0C | 10 pmol | A3 | primer24 | 100 | 2 | 25 | 0.5 | 0.5 | 22 | 50 | MM1 |
NTC (rev) | 0 | A4 | primer24 | 100 | 0 | 25 | 0.5 | 0.5 | 24 | 50 | MM1 |
Sample 0A | 10 pmol | A1 | AmpR6.3-27 | 100 | 2 | 25 | 0.1 | 0.1 | 22.8 | 50 | MM2 |
Sample 0B | 10 pmol | A2 | AmpR6.3-27 | 100 | 2 | 25 | 0.1 | 0.1 | 22.8 | 50 | MM2 |
Sample 0C | 10 pmol | A3 | AmpR6.3-27 | 100 | 2 | 25 | 0.1 | 0.1 | 22.8 | 50 | MM2 |
NTC (rev) | 0 | A4 | AmpR6.3-27 | 100 | 0 | 25 | 0.1 | 0.1 | 24 | 50 | MM2 |
Sample 0A | 10 pmol | A1 | AmpR6.3-28 | 10 | 2 | 25 | 0.5 | 0.5 | 22 | 50 | MM3 |
Sample 0B | 10 pmol | A2 | AmpR6.3-28 | 10 | 2 | 25 | 0.5 | 0.5 | 22 | 50 | MM3 |
Sample 0C | 10 pmol | A3 | AmpR6.3-28 | 10 | 2 | 25 | 0.5 | 0.5 | 22 | 50 | MM3 |
NTC (rev) | 0 | A4 | AmpR6.3-28 | 10 | 0 | 25 | 0.5 | 0.5 | 22 | 50 | MM3 |
Sample 0A | 10 pmol | A1 | AmpR6.3-29 | 10 | 2 | 25 | 0.1 | 0.1 | 22.8 | 50 | MM4 |
Sample 0B | 10 pmol | A2 | AmpR6.3-29 | 10 | 2 | 25 | 0.1 | 0.1 | 22.8 | 50 | MM4 |
Sample 0C | 10 pmol | A3 | AmpR6.3-29 | 10 | 2 | 25 | 0.1 | 0.1 | 22.8 | 50 | MM4 |
NTC (rev) | 0 | A4 | AmpR6.3-29 | 10 | 0 | 25 | 0.1 | 0.1 | 24 | 50 | MM4 |