Daniel:Notebook/ComboLock/2016-10-13
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Padlock0301 Test (Started Friday 10-7)
Library Prep
- Size Select Gel
- Centrifuge tubes at 12000 rpm for 1.5 minutes to bring gel to bottom
- Extract supernatant and add to nanosep column
- Centrifuge at 12000 rpm for 1.5 minutes; collect supernatant
- Ethanol Precipitation
- Add 1250 uL 100% EtOH, 50 uL 3M NaOAc, and 1.5 uL glycoblue to each sample
- Incubate at -80C for a while
- Centrifuge for 25 minutes at 12000 rpm at 4C
- Remove supernatant and add 750 uL 70% EtOH
- Centrifuge for 12 minutes at 12000 rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 30 (20 for NTC) uL nfH2O to resuspend; pool samples A-C by reusing the water
Padlock0301 Dilution Test
Stock Dilutions
Create the following dilutions of PCC Template by taking 10 uL stock and diluting with 90 uL nfH2O (note:zero is normal 10 uM stock). #6 is the highest concentration, made on Friday 10-7.
Tube No | Dilution | Molecules Available | Amount |
6 | 1.00E+06 | 1.00E+06 | 10 amol |
7 | 1.00E+07 | 1.00E+05 | 1 amol |
8 | 1.00E+08 | 1.00E+04 | 100 zmol |
9 | 1.00E+09 | 1000 | 10 zmol |
10 | 1.00E+10 | 100 | 1 zmol |
11 | 1.00E+11 | 10 | 100 ymol |
12 | 1.00E+12 | 1 | 10 ymol |