Daniel:Notebook/ComboLock/2016-10-14
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=Padlock0301 Dilution Test (Started [[Daniel:Notebook/ComboLock/2016-10-14
Protocol
- Circularization
- Heat inactivate enzyme by incubating for 2 minutes at 94C
- Exonuclease Digestion
- Prepare exonuclease I/III mix by mixing 32.5 uL exonuclease I (20 units/μL) and 6.5 uL exonuclease III (100 units/μL) in 1:1 ratio
- Add 2 μL of exonuclease I/III mix to reaction
- Mix the reaction by swirling pipette around the well 5 times
- Incubate reaction at 37 ºC for 2 hours
- Heat inactivate enzyme by incubating at 94C for 5 minutes
- qPCR