Kun:LabNotes/ASE/2007-12-5
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Exp. 1 Perform PCR on the capturing reactions started on 12/03/2007
one reaction 3 reactions Template: 5ul each 10x Buffer: 9.5ul 28.5ul 25mM MgCl2 6ul 18ul 25mM dNTP 1.2ul 3.6ul 100uM AmpSolV6F2 0.4ul 1.2ul 100uM AmpSolV6R2 0.4ul 1.2ul Platinium Taq 1ul 3ul 50X SYBG I 0.8ul 2.4ul H2O 76.7ul 231ul
94C 3min -> 10 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 12 cycles of (94C 45 sec -> 72 1min) -> 72C 3min -> 15C hold.