Kun:LabNotes/ASE/2007-12-5

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Exp. 1 Perform PCR on the capturing reactions started on 12/03/2007

                              one reaction        3 reactions
          Template:              5ul                  each
          10x Buffer:          9.5ul                28.5ul
          25mM MgCl2             6ul                  18ul
          25mM dNTP            1.2ul                 3.6ul
          100uM AmpSolV6F2     0.4ul                 1.2ul
          100uM AmpSolV6R2     0.4ul                 1.2ul
          Platinium Taq          1ul                   3ul
          50X SYBG I           0.8ul                 2.4ul
          H2O                 76.7ul                 231ul

94C 3min -> 10 cycles of (94C 45sec -> 57C 1min -> 72C 1min) -> 12 cycles of (94C 45 sec -> 72 1min) -> 72C 3min -> 15C hold.