Daniel:Notebook/ComboLock/2016-11-3

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Revision as of 18:23, 2 November 2016 by >Djacobse (Created page with " Back to Calendar ==Library Prep== <ol start="3"> <li>Ethanol Precipitation</li> <ol type="A" start="2"> <li>Centrifuge for 25 minutes at 12000...")
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Library Prep

  1. Ethanol Precipitation
    1. Centrifuge for 25 minutes at 12000 rpm at 4C
    2. Remove supernatant and add 750 uL 70% EtOH
    3. Centrifuge for 12 minutes at 12000 rpm at 4C
    4. Remove supernatant and spin in vacuum centrifuge for 10 minutes
    5. Add 30 (20 for NTC) uL nfH2O to resuspend; pool samples A-C by reusing the water