Blue:RNA-Seq Experiments:11072016
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Overview
- Test Smarter v4 chemistry lysis conditions for nuclei
- Initial C1 runs on v4 chemistry showed lower genome mapping rates, therefore it is possible that the new lysis conditions (i.e. 1x lysis buffer) are not optimal for nuclei
- The HT C1 chips run v3 chemistry and have the same lysis conditions, therefore we want to determine whether we can optimize lysis for nuclei
- Test whether polydIdC has any beneficial effect on the new V4 chemistry
Experiment
- Tube control (100 nuclei) test conditions:
- 1x Lysis
- 1x Lysis + 0.1% Triton X-100
- 1x Lysis + 0.2% Triton X-100
- 1x Lysis + PolydidC
- 1x Lysis + 0.1% Triton X-100 + PolydidC
- 1x Lysis + 0.2% Triton X-100 + PolydidC
- 1x Lysis + vortex 30s
- NTC
- Procedure: