Blue:RNA-Seq Experiments:11072016

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Revision as of 17:45, 7 November 2016 by >B1lake (Created page with "== Overview == *Test Smarter v4 chemistry lysis conditions for nuclei **Initial C1 runs on v4 chemistry showed lower genome mapping rates, therefore it is possible that the n...")
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Overview

  • Test Smarter v4 chemistry lysis conditions for nuclei
    • Initial C1 runs on v4 chemistry showed lower genome mapping rates, therefore it is possible that the new lysis conditions (i.e. 1x lysis buffer) are not optimal for nuclei
    • The HT C1 chips run v3 chemistry and have the same lysis conditions, therefore we want to determine whether we can optimize lysis for nuclei
  • Test whether polydIdC has any beneficial effect on the new V4 chemistry


Experiment

  • Tube control (100 nuclei) test conditions:
  1. 1x Lysis
  2. 1x Lysis + 0.1% Triton X-100
  3. 1x Lysis + 0.2% Triton X-100
  4. 1x Lysis + PolydidC
  5. 1x Lysis + 0.1% Triton X-100 + PolydidC
  6. 1x Lysis + 0.2% Triton X-100 + PolydidC
  7. 1x Lysis + vortex 30s
  8. NTC


  • Procedure: