Daniel:Notebook/ComboLock/2016-11-16

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Revision as of 18:01, 16 November 2016 by >Djacobse (Created page with "=BSA Beads Test (Started Yesterday)= Back to Calendar ==Protocol== <li>Rolling Circle Amplification</...")
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BSA Beads Test (Started Yesterday)

Back to Calendar

Protocol

  • Rolling Circle Amplification
    1. Prepare 10.2X Master mix according to table below
    2. Add sample to new reaction tubes according to following table
    3. Reagent uL Added Master Mix (10.2X)
      Template 5 NA
      RCA Primer (100 uM) 2.5 25.5
      dNTP (1 mM) 0.8 8.16
      10X Buffer 2 20.4
      Phi29 1 10.2
      nfH2O 8.7 88.74
      Total 20 153
    4. Incubate at 37C for 3 hours
    5. Incubate at 65C for 10 minutes;Hold at 10C until next step
  • qPCR