Daniel:Notebook/ComboLock/2017-1-7

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Revision as of 19:18, 6 January 2017 by >Djacobse (Created page with "=Version 2 Oligos (Started Yesterday)= Back to Calendar ==Protocol== <ol start="8"> <li>Exonuclease Dig...")
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Version 2 Oligos (Started Yesterday)

Back to Calendar

Protocol

  1. Exonuclease Digestion
    1. Mix 12.5 uL Exo I (20U/uL) and 2.5 uL ExoIII (100U/uL)
    2. Add 2 uL to each sample, mix by swirling pipette tip
    3. Incubate for 1 hour at 37C
    4. Heat kill by incubating for 5 min at 95C
  2. Rolling Circle Amplification
    1. Prepare 7X Master mix according to table below
    2. Add 15 uL master mix to each tube
    3. Add sample to new reaction tubes according to following table