Daniel:Notebook/ComboLock/2017-1-10

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Revision as of 21:54, 13 January 2017 by >Djacobse (→‎Library Prep (Started Yesterday))
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Version 2 Oligos (Started 1/6/2017)

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Library Prep (Started Yesterday)

  1. Ethanol Precipitation
    1. Centrifuge for 25 minutes at 12000 rpm at 4C
    2. Remove supernatant and add 750 uL chilled 70% EtOH
    3. Centrifuge for 12 minutes at 12000 rpm at 4C
    4. Remove supernatant and spin in vacuum centrifuge for 10 minutes
    5. Add 30 uL nfH2O to resuspend; pool samples A and B by reusing the water
Sample ng/uL ug in 30 uL
Sample Lanes (1A+1B) 40.3 1.2

DEJ20170109-CLv2-PC