Daniel:Notebook/ComboLock/2017-1-10
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Version 2 Oligos (Started 1/6/2017)
Library Prep (Started Yesterday)
- Ethanol Precipitation
- Centrifuge for 25 minutes at 12000 rpm at 4C
- Remove supernatant and add 750 uL chilled 70% EtOH
- Centrifuge for 12 minutes at 12000 rpm at 4C
- Remove supernatant and spin in vacuum centrifuge for 10 minutes
- Add 30 uL nfH2O to resuspend; pool samples A and B by reusing the water
Sample | ng/uL | ug in 30 uL |
Sample Lanes (1A+1B) | 40.3 | 1.2 |
DEJ20170109-CLv2-PC